PAX6 Antibody
Catalog Number: LS-C163391-400
400 µL / $401.00
Antibody: Mouse Monoclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PAX6 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G77901-20
Catalog Number: LS-G77901
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PAX6 (NM_001604) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AN2 | D11S812E | MGDA | Oculorhombin | Paired box 6 | Paired box homeotic gene-6 | PAX6 | AN | Aniridia type II protein | WAGR | Paired box protein Pax-6
- Origin Species
- Human
- NCBI GenBank
- NM_001604
- SwissProt
- P26367
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQNSHSGVNQ LGGVFVNGRP LPDSTRQKIV ELAHSGARPC DISRILQTHA DAKVQVLDNQ61NVSNGCVSKI LGRYYETGSI RPRAIGGSKP RVATPEVVSK IAQYKRECPS IFAWEIRDRL121LSEGVCTNDN IPSVSSINRV LRNLASEKQQ MGADGMYDKL RMLNGQTGSW GTRPGWYPGT181SVPGQPTQDG CQQQEGGGEN TNSISSNGED SDEAQMRLQL KRKLQRNRTS FTQEQIEALE241KEFERTHYPD VFARERLAAK IDLPEARIQV WFSNRRAKWR REEKLRNQRR QASNTPSHIP301ISSSFSTSVY QPIPQPTTPV SSFTSGSMLG RTDTALTNTY SALPPMPSFT MANNLPMQPP361VPSQTSSYSC MLPTSPSVNG RSYDTYTPPH MQTHMNSQPM GTSGTTSTGL ISPGVSVPVQ421VPGSEPDMSQ YWPRLQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
436 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
