PAPL Antibody (aa378-413)
Catalog Number: LS-C344694-400
400 µL / $401.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Zebrafish
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PAPL Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G96147-20
Catalog Number: LS-G96147
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PAPL(PAPL) (NM_001004318) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001004318
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MHPLPGYWSC YCLLLLFSLG VQGSLGAPSA APEQVHLSYP GEPGSMTVTW TTWVPTRSEV61QFGLQPSGPL PLRAQGTFVP FVDGGILRRK LYIHRVTLRK LLPGVQYVYR CGSAQGWSRR121FRFRALKNGA HWSPRLAVFG DLGADNPKAV PRLRRDTQQG MYDAVLHVGD FAYNLDQDNA181RVGDRFMRLI EPVAASLPYM TCPGNHEERY NFSNYKARFS MPGDNEGLWY SWDLGPAHII241SFSTEVYFFL HYGRHLVQRQ FRWLESDLQK ANKNRAARPW IITMGHRPMY CSNADLDDCT301RHESKVRKGL QGKLYGLEDL FYKYGVDLQL WAHEHSYERL WPIYNYQVFN GSREMPYTNP361RGPVHIITGS AGCEERLTPF AVFPRPWSAV RVKEYGYTRL HILNGTHIHI QQVSDDQDGK421IVDDVWVVRP LFGRRMYLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
438 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
