PAK1 Antibody
Catalog Number: LS-C488631-100
100 µL / $309.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PAK1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G78762-20
Catalog Number: LS-G78762
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PAK1 (NM_001128620) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha-PAK | p21-activated kinase 1 | p65-PAK | PAK1 | Rac/p21-activated kinase | STE20 homolog | yeast | PAKalpha | PAK-1
- Origin Species
- Human
- NCBI GenBank
- NM_002576
- SwissProt
- Q13153
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSNNGLDIQD KPPAPPMRNT STMIGAGSKD AGTLNHGSKP LPPNPEEKKK KDRFYRSILP61GDKTNKKKEK ERPEISLPSD FEHTIHVGFD AVTGEFTGMP EQWARLLQTS NITKSEQKKN121PQAVLDVLEF YNSKKTSNSQ KYMSFTDKSA EDYNSSNALN VKAVSETPAV PPVSEDEDDD181DDDATPPPVI APRPEHTKSV YTRSVIEPLP VTPTRDVATS PISPTENNTT PPDALTRNTE241KQKKKPKMSD EEILEKLRSI VSVGDPKKKY TRFEKIGQGA SGTVYTAMDV ATGQEVAIKQ301MNLQQQPKKE LIINEILVMR ENKNPNIVNY LDSYLVGDEL WVVMEYLAGG SLTDVVTETC361MDEGQIAAVC RECLQALEFL HSNQVIHRDI KSDNILLGMD GSVKLTDFGF CAQITPEQSK421RSTMVGTPYW MAPEVVTRKA YGPKVDIWSL GIMAIEMIEG EPPYLNENPL RALYLIATNG481TPELQNPEKL SAIFRDFLNR CLEMDVEKRG SAKELLQVRK LRFQVFSNFS MIAASIPEDC541QAPLQPHSTD CCSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
553 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
