LSBio
Human P2RX6 / P2X6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human P2RX6 / P2X6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all P2RX6 antibodies →
$153.00each
Catalog Number: LS-G79723-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human P2X6(P2RX6) (NM_001159554) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATP receptor | p2RXL1 | p2X purinoceptor 6 | p2RX6 | p2X6 | p2X6 purinoceptor | p2XM | Purinergic receptor | Purinoreceptor P2X6 | Skeletal muscle-expressed P2X | p2X receptor 6 | p2X-like 1 | Purinergic receptor P2X-like 1 | Purinoceptor P2X6
Origin Species
Human
NCBI GenBank
NM_005446
SwissProt
O15547

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
45.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MCPQLAGAGS MGSPGATTGW GLLDYKTEKW ALLAKKGYQE RDLEPQFSII TKLKGVSVTQ
61IKELGNRLWD VADFVKPPQG ENVFFLVTNF LVTPAQVQGR CPEHPSVPLA NCWVDEDCPE
121GEGGTHSHGV KTGQCVVFNG THRTCEIWSW CPVESGVVPS RPLLAQAQNF TLFIKNTVTF
181SKFNFSKSNA LETWDPTYFK HCRYEPQFSP YCPVFRIGDL VAKAGGTFED LALLGGSVGI
241RVHWDCDLDT GDSGCWPHYS FQLQEKSYNF RTATHWWEQP GVEARTLLKL YGIRFDILVT
301GQAGKFGLIP TAVTLGTGAA WLGVVTFFCD LLLLYVDREA HFYWRTKYEE AKAPKATANS
361VWRELALASQ ARLAECLRRS SAPAPTATAA GSQTQTPGWP CPSSDTHLPT HSGSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

415 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.