OSBP Antibody
Catalog Number: LS-C663249
6 sizes / from $316.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human OSBP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G77853-20
Catalog Number: LS-G77853
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human OSBP1(OSBP) (NM_002556) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- OSBP1 | Oxysterol binding protein | OSBP | Oxysterol-binding protein 1
- Origin Species
- Human
- NCBI GenBank
- NM_002556
- SwissProt
- P22059
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 89.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAATELRGVV GPGPAAIAAL GGGGAGPPVV GGGGGRGDAG PGSGAASGTV VAAAAGGPGP61GAGGVAAAGP APAPPTGGSG GSGAGGSGSA REGWLFKWTN YIKGYQRRWF VLSNGLLSYY121RSKAEMRHTC RGTINLATAN ITVEDSCNFI ISNGGAQTYH LKASSEVERQ RWVTALELAK181AKAVKMLAES DESGDEESVS QTDKTELQNT LRTLSSKVED LSTCNDLIAK HGTALQRSLS241ELESLKLPAE SNEKIKQVNE RATLFRITSN AMINACRDFL MLAQTHSKKW QKSLQYERDQ301RIRLEETLEQ LAKQHNHLER AFRGATVLPA NTPGNVGSGK DQCCSGKGDM SDEDDENEFF361DAPEIITMPE NLGHKRTGSN ISGASSDISL DEQYKHQLEE TKKEKRTRIP YKPNYSLNLW421SIMKNCIGKE LSKIPMPVNF NEPLSMLQRL TEDLEYHELL DRAAKCENSL EQLCYVAAFT481VSSYSTTVFR TSKPFNPLLG ETFELDRLEE NGYRSLCEQV SHHPPAAAHH AESKNGWTLR541QEIKITSKFR GKYLSIMPLG TIHCIFHATG HHYTWKKVTT TVHNIIVGKL WIDQSGEIDI601VNHKTGDKCN LKFVPYSYFS RDVARKVTGE VTDPSGKVHF ALLGTWDEKM ECFKVQPVIG661ENGGDARQRG HEAEESRVML WKRNPLPKNA ENMYYFSELA LTLNAWESGT APTDSRLRPD721QRLMENGRWD EANAEKQRLE EKQRLSRKKR EAEAMKATED GTPYDPYKAL WFERKKDPVT781KELTHIYRGE YWECKEKQDW SSCPDIFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
807 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
