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Human OR10A5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human OR10A5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all OR10A5 antibodies →
$153.00each
Catalog Number: LS-G89083-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human OR10A5 (NM_178168) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HP3 | OR10A1 | Olfactory receptor 10A5 | Olfactory receptor 11-403 | Olfactory receptor OR11-85 | JCG6 | Olfactory receptor 10A1 | OR10A5 | OR11-403
Origin Species
Human
NCBI GenBank
AF324499
SwissProt
Q9H207

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
35.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MAIGNWTEIS EFILMSFSSL PTEIQSLLFL TFLTIYLVTL KGNSLIILVT LADPMLHSPM
61YFFLRNLSFL EIGFNLVIVP KMLGTLLAQD TTISFLGCAT QMYFFFFFGV AECFLLATMA
121YDRYVAICSP LHYPVIMNQR TRAKLAAASW FPGFPVATVQ TTWLFSFPFC GTNKVNHFFC
181DSPPVLKLVC ADTALFEIYA IVGTILVVMI PCLLILCSYT RIAAAILKIP SAKGKHKAFS
241TCSSHLLVVS LFYISSSLTY FWPKSNNSPE SKKLLSLSYT VVTPMLNPII YSLRNSEVKN
301ALSRTFHKVL ALRNCIP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

317 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.