LSBio
Human NUP85 / Pericentrin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NUP85 / Pericentrin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NUP85 antibodies →
$153.00each
Catalog Number: LS-G86531-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Pericentrin 1(NUP85) (NM_024844) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
85 kDa nucleoporin | Frount | Nucleoporin 85kDa | Nucleoporin Nup75 | Nucleoporin Nup85 | Nup75 | PCNT1 | Pericentrin-1 | NUP85
Origin Species
Human
NCBI GenBank
NM_024844
SwissProt
Q9BW27

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MEELDGEPTV TLIPGVNSKK NQMYFDWGPG EMLVCETSFN KKEKSEMVPS CPFIYIIRKD
61VDVYSQILRK LFNESHGIFL GLQRIDEELT GKSRKSQLVR VSKNYRSVIR ACMEEMHQVA
121IAAKDPANGR QFSSQVSILS AMELIWNLCE ILFIEVAPAG PLLLHLLDWV RLHVCEVDSL
181SADVLGSENP SKHDSFWNLV TILVLQGRLD EARQMLSKEA DASPASAGIC RIMGDLMRTM
241PILSPGNTQT LTELELKWQH WHEECERYLQ DSTFATSPHL ESLLKIMLGD EAALLEQKEL
301LSNWYHFLVT RLLYSNPTVK PIDLHYYAQS SLDLFLGGES SPEPLDNILL AAFEFDIHQV
361IKECSIALSN WWFVAHLTDL LDHCKLLQSH NLYFGSNMRE FLLLEYASGL FAHPSLWQLG
421VDYFDYCPEL GRVSLELHIE RIPLNTEQKA LKVLRICEQR QMTEQVRSIC KILAMKAVRN
481NRLGSALSWS IRAKDAAFAT LVSDRFLRDY CERGCFSDLD LIDNLGPAMM LSDRLTFLGK
541YREFHRMYGE KRFADAASLL LSLMTSRIAP RSFWMTLLTD ALPLLEQKQV IFSAEQTYEL
601MRCLEDLTSR RPVHGESDTE QLQDDDIETT KVEMLRLSLA RNLARAIIRE GSLEGS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

656 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.