LSBio
Human NTRK2 / TRKB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NTRK2 / TRKB Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NTRK2 antibodies →
$220.00each
Catalog Number: LS-G74731-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TrkB(NTRK2) (NM_006180) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
gp145-TrkB | Trk-B | TRKB | Tropomyosin-related kinase B | NTRK2 | TrkB tyrosine kinase | Tyrosine kinase receptor B
Origin Species
Human
NCBI GenBank
NM_006180
SwissProt
Q16620

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.83 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSWIRWHGP AMARLWGFCW LVVGFWRAAF ACPTSCKCSA SRIWCSDPSP GIVAFPRLEP
61NSVDPENITE IFIANQKRLE IINEDDVEAY VGLRNLTIVD SGLKFVAHKA FLKNSNLQHI
121NFTRNKLTSL SRKHFRHLDL SELILVGNPF TCSCDIMWIK TLQEAKSSPD TQDLYCLNES
181SKNIPLANLQ IPNCGLPSAN LAAPNLTVEE GKSITLSCSV AGDPVPNMYW DVGNLVSKHM
241NETSHTQGSL RITNISSDDS GKQISCVAEN LVGEDQDSVN LTVHFAPTIT FLESPTSDHH
301WCIPFTVKGN PKPALQWFYN GAILNESKYI CTKIHVTNHT EYHGCLQLDN PTHMNNGDYT
361LIAKNEYGKD EKQISAHFMG WPGIDDGANP NYPDVIYEDY GTAANDIGDT TNRSNEIPST
421DVTDKTGREH LSVYAVVVIA SVVGFCLLVM LFLLKLARHS KFGMKDFSWF GFGKVKSRQG
481VGPASVISND DDSASPLHHI SNGSNTPSSS EGGPDAVIIG MTKIPVIENP QYFGITNSQL
541KPDTFVQHIK RHNIVLKREL GEGAFGKVFL AECYNLCPEQ DKILVAVKTL KDASDNARKD
601FHREAELLTN LQHEHIVKFY GVCVEGDPLI MVFEYMKHGD LNKFLRAHGP DAVLMAEGNP
661PTELTQSQML HIAQQIAAGM VYLASQHFVH RDLATRNCLV GENLLVKIGD FGMSRDVYST
721DYYRVGGHTM LPIRWMPPES IMYRKFTTES DVWSLGVVLW EIFTYGKQPW YQLSNNEVIE
781CITQGRVLQR PRTCPQEVYE LMLGCWQREP HMRKNIKGIH TLLQNLAKAS PVYLDILG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

838 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.