LSBio
Human NOL10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NOL10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NOL10 antibodies →
$220.00each
Catalog Number: LS-G85430-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NOL10 (NM_024894) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
H_NH0074G24.1 | NOL10 | Nucleolar protein 10 | PQBP5
Origin Species
Human
NCBI GenBank
NM_024894
SwissProt
Q9BSC4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQVSSLNEVK IYSLSCGKSL PEWLSDRKKR ALQKKDVDVR RRIELIQDFE MPTVCTTIKV
61SKDGQYILAT GTYKPRVRCY DTYQLSLKFE RCLDSEVVTF EILSDDYSKI VFLHNDRYIE
121FHSQSGFYYK TRIPKFGRDF SYHYPSCDLY FVGASSEVYR LNLEQGRYLN PLQTDAAENN
181VCDINSVHGL FATGTIEGRV ECWDPRTRNR VGLLDCALNS VTADSEINSL PTISALKFNG
241ALTMAVGTTT GQGKIFTSLE PEHDLNDVCL YPNSGMLLTA NETPKMGIYY IPVLGPAPRW
301CSFLDNLTEE LEENPESTVY DDYKFVTKKD LENLGLTHLI GSPFLRAYMH GFFMDIRLYH
361KVKLMVNPFA YEEYRKDKIR QKIEETRAQR VQLKKLPKVN KELALKLIEE EEEKQKSTWK
421KKVKSLPNIL TDDRFKVMFE NPDLQVDEES EEFRLLNPLV SKISEKRKKK LRLLEQQELR
481EKEEEEEPEG KPSDAESSES SDDEKAWVEE VRKQRRLLQR EEKVKRQERL KEDQQTVLKP
541QFYEIKAGEE FRSFKDSATK QKLMNKTLED RLKIEAKNGT LSVSDTTVGS KQLTFTLKRS
601EQQKKQQEAE KLHRQERKRL RRSAGHLKSR HKRGRSFH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

638 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.