LSBio
Human NOD8 / NLRP10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NOD8 / NLRP10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NLRP10 antibodies →
$153.00each
Catalog Number: LS-G94168-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NLRP10 (NM_176821) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLR11.1 | NLRP10 | PYNOD | NALP10 | NOD8 | PAN5
Origin Species
Human
SwissProt
Q86W26

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAMAKARKPR EALLWALSDL EENDFKKLKF YLRDMTLSEG QPPLARGELE GLIPVDLAEL
61LISKYGEKEA VKVVLKGLKV MNLLELVDQL SHICLHDYRE VYREHVRCLE EWQEAGVNGR
121YNQVLLVAKP SSESPESLAC PFPEQELESV TVEALFDSGE KPSLAPSLVV LQGSAGTGKT
181TLARKMVLDW ATGTLYPGRF DYVFYVSCKE VVLLLESKLE QLLFWCCGDN QAPVTEILRQ
241PERLLFILDG FDELQRPFEE KLKKRGLSPK ESLLHLLIRR HTLPTCSLLI TTRPLALRNL
301EPLLKQARHV HILGFSEEER ARYFSSYFTD EKQADRAFDI VQKNDILYKA CQVPGICWVV
361CSWLQGQMER GKVVLETPRN STDIFMAYVS TFLPPDDDGG CSELSRHRVL RSLCSLAAEG
421IQHQRFLFEE AELRKHNLDG PRLAAFLSSN DYQLGLAIKK FYSFRHISFQ DFFHAMSYLV
481KEDQSRLGKE SRREVQRLLE VKEQEGNDEM TLTMQFLLDI SKKDSFSNLE LKFCFRISPC
541LAQDLKHFKE QMESMKHNRT WDLEFSLYEA KIKNLVKGIQ MNNVSFKIKH SNEKKSQSQN
601LFSVKSSLSH GPKEEQKCPS VHGQKEGKDN IAGTQKEAST GKGRGTEETP KNTYI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

655 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.