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Human NFX1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NFX1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NFX1 antibodies →
$153.00each
Catalog Number: LS-G79269-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NFX1 (NM_002504) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NFX1 | NFX2
Origin Species
Human
NCBI GenBank
NM_002504
SwissProt
Q12986

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEAPPVSGT FKFNTDAAEF IPQEKKNSGL NCGTQRRLDS NRIGRRNYSS PPPCHLSRQV
61PYDEISAVHQ HSYHPSGSKP KSQQTSFQSS PCNKSPKSHG LQNQPWQKLR NEKHHIRVKK
121AQSLAEQTSD TAGLESSTRS ESGTDLREHS PSESEKEVVG ADPRGAKPKK ATQFVYSYGR
181GPKVKGKLKC EWSNRTTPKP EDAGPESTKP VGVFHPDSSE ASSRKGVLDG YGARRNEQRR
241YPQKRPPWEV EGARPRPGRN PPKQEGHRHT NAGHRNNMGP IPKDDLNERP AKSTCDSENL
301AVINKSSRRV DQEKCTVRRQ DPQVVSPFSR GKQNHVLKNV ETHTGSLIEQ LTTEKYECMV
361CCELVRVTAP VWSCQSCYHV FHLNCIKKWA RSPASQADGQ SGWRCPACQN VSAHVPNTYT
421CFCGKVKNPE WSRNEIPHSC GEVCRKKQPG QDCPHSCNLL CHPGPCPPCP AFMTKTCECG
481RTRHTVRCGQ AVSVHCSNPC ENILNCGQHQ CAELCHGGQC QPCQIILNQV CYCGSTSRDV
541LCGTDVGKSD GFGDFSCLKI CGKDLKCGNH TCSQVCHPQP CQQCPRLPQL VRCCPCGQTP
601LSQLLELGSS SRKTCMDPVP SCGKVCGKPL PCGSLDFIHT CEKLCHEGDC GPCSRTSVIS
661CRCSFRTKEL PCTSLKSEDA TFMCDKRCNK KRLCGRHKCN EICCVDKEHK CPLICGRKLR
721CGLHRCEEPC HRGNCQTCWQ ASFDELTCHC GASVIYPPVP CGTRPPECTQ TCARVHECDH
781PVYHSCHSEE KCPPCTFLTQ KWCMGKHEFR SNIPCHLVDI SCGLPCSATL PCGMHKCQRL
841CHKGECLVDE PCKQPCTTPR ADCGHPCMAP CHTSSPCPVT ACKAKVELQC ECGRRKEMVI
901CSEASSTYQR IAAISMASKI TDMQLGGSVE ISKLITKKEV HQARLECDEE CSALERKKRL
961AEAFHISEDS DPFNIRSSGS KFSDSLKEDA RKDLKFVSDV EKEMETLVEA VNKGKNSKKS
1021HSFPPMNRDH RRIIHDLAQV YGLESVSYDS EPKRNVVVTA IRGKSVCPPT TLTGVLEREM
1081QARPPPPIPH HRHQSDKNPG SSNLQKITKE PIIDYFDVQD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1120 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.