LSBio
Human NEPH1 / KIRREL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NEPH1 / KIRREL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KIRREL antibodies →
$153.00each
Catalog Number: LS-G90881-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KIRREL (NM_018240) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIRREL1 | KIRREL | Nephrin-like protein 1 | Kin of IRRE-like protein 1 | Kin of IRRE like (Drosophila) | NEPH1 | Nephrin related
Origin Species
Human
NCBI GenBank
NM_018240
SwissProt
Q96J84

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLSLLVWILT LSDTFSQGTQ TRFSQEPADQ TVVAGQRAVL PCVLLNYSGI VQWTKDGLAL
61GMGQGLKAWP RYRVVGSADA GQYNLEITDA ELSDDASYEC QATEAALRSR RAKLTVLIPP
121EDTRIDGGPV ILLQAGTPHN LTCRAFNAKP AATIIWFRDG TQQEGAVAST ELLKDGKRET
181TVSQLLINPT DLDIGRVFTC RSMNEAIPSG KETSIELDVH HPPTVTLSIE PQTVQEGERV
241VFTCQATANP EILGYRWAKG GFLIEDAHES RYETNVDYSF FTEPVSCEVH NKVGSTNVST
301LVNVHFAPRI VVDPKPTTTD IGSDVTLTCV WVGNPPLTLT WTKKDSNMVL SNSNQLLLKS
361VTQADAGTYT CRAIVPRIGV AEREVPLYVN GPPIISSEAV QYAVRGDGGK VECFIGSTPP
421PDRIAWAWKE NFLEVGTLER YTVERTNSGS GVLSTLTINN VMEADFQTHY NCTAWNSFGP
481GTAIIQLEER EVLPVGIIAG ATIGASILLI FFFIALVFFL YRRRKGSRKD VTLRKLDIKV
541ETVNREPLTM HSDREDDTAS VSTATRVMKA IYSSFKDDVD LKQDLRCDTI DTREEYEMKD
601PTNGYYNVRA HEDRPSSRAV LYADYRAPGP ARFDGRPSSR LSHSSGYAQL NTYSRGPASD
661YGPEPTPPGP AAPAGTDTTS QLSYENYEKF NSHPFPGAAG YPTYRLGYPQ APPSGLERTP
721YEAYDPIGKY ATATRFSYTS QHSDYGQRFQ QRMQTHV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

757 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.