NEDD8 Antibody
Catalog Number: LS-C192417
2 sizes / from $364.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Human NEDD8 Protein (Recombinant His)
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| Expression System | Escherichia coli (Prokaryotic) |
| Format: | Liquid |
$267.00each
Catalog Number: LS-G3502-20
Catalog Number: LS-G3502
For bulk orders or custom formulations:
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- Description
- NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E.coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.
- Category
- Proteins & Peptides > Proteins > Recombinant Proteins
- Alternative Names
- NEDD8 | NEDD-8 | Neddylin | Ubiquitin-like protein Nedd8
- Origin Species
- Human
- NCBI GenBank
- NM_006156|NP_006147.1
- SwissProt
- Q15843
Protein
- Expression System
- Escherichia coli (Prokaryotic)
- Fusion Tag
- Hexahistidine Tag
- Protein Type
- Recombinant
- Amino Acid Sequence
11-76aa|MRG SHHHHHHGMA SMTGGQQMGR DLYDDDDKDR WGSHMLIKVK TLTGKEIEID61IEPTDKVERI KERVEEKEGI PPQQQRLIYS GKQMNDEKTA ADYKILGGSV LHLVLALRGG
120 aa
Format
- Purity
- >95%
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- < 1.0 EU/µg
- Format
- Liquid
- Formulation
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 50mM NaCl, 10% glycerol.
- Purity Measurement Method
- SDS-PAGE (Coomassie Stain)
Storage & Handling
- Storage Conditions
- Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.
