NDUFV3 Antibody
Catalog Number: LS-C808943
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NDUFV3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G77585-20
Catalog Number: LS-G77585
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NDUFV3 (NM_021075) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CI-9KD | CI-10k | NDUFV3 | Complex I 10kDa subunit | Complex I-9kD
- Origin Species
- Human
- NCBI GenBank
- NM_021075
- SwissProt
- P56181
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPCLLRQG RAGALKTMLQ EAQVFRGLAS TVSLSAESGK SEKGQPQNSK KQSPPKNVVE61PKERGKLLAT QTAAELSKNL SSPSSYPPAV NKGRKVASPS PSGSVLFTDE GVPKFLSRKT121LVEFPQKVLS PFRKQGSDSE ARQVGRKVTS PSSSSSSSSS DSESDDEADV SEVTPRVVSK181GRGGLRKPEA SHSFENRAPR VTVSAKEKTL LQKPHVDITD PEKPHQPKKK GSPAKPSEGR241ENARPKTTMP RSQVDEEFLK QSLKEKQLQK TFRLNEIDKE SQKPFEVKGP LPVHTKSGLS301APPKGSPAPA VLAEEARAEG QLQASPPGAA EGHLEKPVPE PQRKAAPPLP RKETSGTQGI361EGHLKGGQAI VEDQIPPSNL ETVPVENNHG FHEKTAALKL EAEGEAMEDA AAPGNDRGGT421QEPAPVPAEP FDNTTYKNLQ HHDYSTYTFL DLNLELSKFR MPQPSSGRES PRHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
473 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
