LSBio
Human NBEA / Neurobeachin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NBEA / Neurobeachin Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NBEA antibodies →
$220.00each
Catalog Number: LS-G90157-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Neurobeachin(NBEA) (NM_015678) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LYST2 | Neurobeachin | Protein BCL8B | BCL8B | KIAA1544 | NBEA
Origin Species
Human
NCBI GenBank
NM_015678
SwissProt
Q8NFP9

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
327.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASEKPGPGP GLEPQPVGLI AVGAAGGGGG GSGGGGTGGS GMGELRGASG SGSVMLPAGM
61INPSVPIRNI RMKFAVLIGL IQVGEVSNRD IVETVLNLLV GGEFDLEMNF IIQDAESITC
121MTELLEHCDV TCQAEIWSMF TAILRKSVRN LQTSTEVGLI EQVLLKMSAV DDMIADLLVD
181MLGVLASYSI TVKELKLLFS MLRGESGIWP RHAVKLLSVL NQMPQRHGPD TFFNFPGCSA
241AAIALPPIAK WPYQNGFTLN TWFRMDPLNN INVDKDKPYL YCFRTSKGVG YSAHFVGNCL
301IVTSLKSKGK GFQHCVKYDF QPRKWYMISI VHIYNRWRNS EIRCYVNGQL VSYGDMAWHV
361NTNDSYDKCF LGSSETADAN RVFCGQLGAV YVFSEALNPA QIFAIHQLGP GYKSTFKFKS
421ESDIHLAEHH KQVLYDGKLA SSIAFTYNAK ATDAQLCLES SPKENASIFV HSPHALMLQD
481VKAIVTHSIH SAIHSIGGIQ VLFPLFAQLD NRQLNDSQVE TTVCATLLAF LVELLKSSVA
541MQEQMLGGKG FLVIGYLLEK SSRVHITRAV LEQFLSFAKY LDGLSHGAPL LKQLCDHILF
601NPAIWIHTPA KVQLSLYTYL SAEFIGTATI YTTIRRVGTV LQLMHTLKYY YWVINPADSS
661GITPKGLDGP RPSQKEIISL RAFMLLFLKQ LILKDRGVKE DELQSILNYL LTMHEDENIH
721DVLQLLVALM SEHPASMIPA FDQRNGIRVI YKLLASKSES IWVQALKVLG YFLKHLGHKR
781KVEIMHTHSL FTLLGERLML HTNTVTVTTY NTLYEILTEQ VCTQVVHKPH PEPDSTVKIQ
841NPMILKVVAT LLKNSTPSAE LMEVRRLFLS DMIKLFSNSR ENRRCLLQCS VWQDWMFSLG
901YINPKNSEEQ KITEMVYNIF RILLYHAIKY EWGGWRVWVD TLSIAHSKVT YEAHKEYLAK
961MYEEYQRQEE ENIKKGKKGN VSTISGLSSQ TTGAKGGMEI REIEDLSQSQ SPESETDYPV
1021STDTRDLLMS TKVSDDILGN SDRPGSGVHV EVHDLLVDIK AEKVEATEVK LDDMDLSPET
1081LVGGENGALV EVESLLDNVY SAAVEKLQNN VHGSVGIIKK NEEKDNGPLI TLADEKEDLP
1141NSSTSFLFDK IPKQEEKLLP ELSSNHIIPN IQDTQVHLGV SDDLGLLAHM TGSVDLTCTS
1201SIIEEKEFKI HTTSDGMSSI SERDLASSTK GLEYAEMTAT TLETESSSSK IVPNIDAGSI
1261ISDTERSDDG KESGKEIRKI QTTTTTQAVQ GRSITQQDRD LRVDLGFRGM PMTEEQRRQF
1321SPGPRTTMFR IPEFKWSPMH QRLLTDLLFA LETDVHVWRS HSTKSVMDFV NSNENIIFVH
1381NTIHLISQMV DNIIIACGGI LPLLSAATSP TGSKTELENI EVTQGMSAET AVTFLSRLMA
1441MVDVLVFASS LNFSEIEAEK NMSSGGLMRQ CLRLVCCVAV RNCLECRQRQ RDRGNKSSHG
1501SSKPQEVPQS VTATAASKTP LENVPGNLSP IKDPDRLLQD VDINRLRAVV FRDVDDSKQA
1561QFLALAVVYF ISVLMVSKYR DILEPQRETT RTGSQPGRNI RQEINSPTST VVVIPSIPHP
1621SLNHGFLAKL IPEQSFGHSF YKETPAAFPD TIKEKETPTP GEDIQVESSI PHTDSGIGEE
1681QVASILNGAE LETSTGPDAM SELLSTLSSE VKKSQESLTE NPSETLKPAT SISSISQTKG
1741INVKEILKSL VAAPVEIAEC GPEPIPYPDP ALKRETQAIL PMQFHSFDRS VVVPVKKPPP
1801GSLAVTTVGA TTAGSGLPTG STSNIFAATG ATPKSMINTT GAVDSGSSSS SSSSSFVNGA
1861TSKNLPAVQT VAPMPEDSAE NMSITAKLER ALEKVAPLLR EIFVDFAPFL SRTLLGSHGQ
1921ELLIEGLVCM KSSTSVVELV MLLCSQEWQN SIQKNAGLAF IELINEGRLL CHAMKDHIVR
1981VANEAEFILN RQRAEDVHKH AEFESQCAQY AADRREEEKM CDHLISAAKH RDHVTANQLK
2041QKILNILTNK HGAWGAVSHS QLHDFWRLDY WEDDLRRRRR FVRNAFGSTH AEALLKAAIE
2101YGTEEDVVKS KKTFRSQAIV NQNAETELML EGDDDAVSLL QEKEIDNLAG PVVLSTPAQL
2161IAPVVVAKGT LSITTTEIYF EVDEDDSAFK KIDTKVLAYT EGLHGKWMFS EIRAVFSRRY
2221LLQNTALEVF MANRTSVMFN FPDQATVKKV VYSLPRVGVG TSYGLPQARR ISLATPRQLY
2281KSSNMTQRWQ RREISNFEYL MFLNTIAGRT YNDLNQYPVF PWVLTNYESE ELDLTLPGNF
2341RDLSKPIGAL NPKRAVFYAE RYETWEDDQS PPYHYNTHYS TATSTLSWLV RIEPFTTFFL
2401NANDGKFDHP DRTFSSVARS WRTSQRDTSD VKELIPEFYY LPEMFVNSNG YNLGVREDEV
2461VVNDVDLPPW AKKPEDFVRI NRMALESEFV SCQLHQWIDL IFGYKQRGPE AVRALNVFHY
2521LTYEGSVNLD SITDPVLREA MEAQIQNFGQ TPSQLLIEPH PPRSSAMHLC FLPQSPLMFK
2581DQMQQDVIMV LKFPSNSPVT HVAANTLPHL TIPAVVTVTC SRLFAVNRWH NTVGLRGAPG
2641YSLDQAHHLP IEMDPLIANN SGVNKRQITD LVDQSIQINA HCFVVTADNR YILICGFWDK
2701SFRVYSTETG KLTQIVFGHW DVVTCLARSE SYIGGDCYIV SGSRDATLLL WYWSGRHHII
2761GDNPNSSDYP APRAVLTGHD HEVVCVSVCA ELGLVISGAK EGPCLVHTIT GDLLRALEGP
2821ENCLFPRLIS VSSEGHCIIY YERGRFSNFS INGKLLAQME INDSTRAILL SSDGQNLVTG
2881GDNGVVEVWQ ACDFKQLYIY PGCDAGIRAM DLSHDQRTLI TGMASGSIVA FNIDFNRWHY
2941EHQNRY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2946 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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