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Human NALP4 / NLRP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NALP4 / NLRP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G92624-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NALP4(NLRP4) (NM_134444) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 58 | CLR19.5 | CT58 | NLRP4 | PYPAF4 | RNH2 | NALP4 | Ribonuclease inhibitor 2
Origin Species
Human
SwissProt
Q96MN2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAASFFSDFG LMWYLEELKK EEFRKFKEHL KQMTLQLELK QIPWTEVKKA SREELANLLI
61KHYEEQQAWN ITLRIFQKMD RKDLCMKVMR ERTGYTKTYQ AHAKQKFSRL WSSKSVTEIH
121LYFEEEVKQE ECDHLDRLFA PKEAGKQPRT VIIQGPQGIG KTTLLMKLMM AWSDNKIFRD
181RFLYTFYFCC RELRELPPTS LADLISREWP DPAAPITEIV SQPERLLFVI DSFEELQGGL
241NEPDSDLCGD LMEKRPVQVL LSSLLRKKML PEASLLIAIK PVCPKELRDQ VTISEIYQPR
301GFNESDRLVY FCCFFKDPKR AMEAFNLVRE SEQLFSICQI PLLCWILCTS LKQEMQKGKD
361LALTCQSTTS VYSSFVFNLF TPDGAEGPTQ QTQHQLKALC SLAAEGMWTD TFEFCEDDLR
421RNGVVDADIP ALLGTKILLK YGERESSYVF LHVCIQEFCA ALFYLLKSHL DHPHPAVRCV
481QELLVANFEK ARRAHWIFLG CFLTGLLNKK EQEKLDAFFG FQLSQEIKQQ IHQCLKSLGE
541RGNPQGQVDS LAIFYCLFEM QDPAFVKQAV NLLQEANFHI IDNVDLVVSA YCLKYCSSLR
601KLCFSVQNVF KKEDEHSSTS DYSLICWHHI CSVLTTSGHL RELQVQDSTL SESTFVTWCN
661QLRHPSCRLQ KLGINNVSFS GQSVLLFEVL FYQPDLKYLS FTLTKLSRDD IRSLCDALNY
721PAGNVKELAL VNCHLSPIDC EVLAGLLTNN KKLTYLNVSC NQLDTGVPLL CEAMCSPDTV
781LVYLMLAFCH LSEQCCEYIS EMLLRNKSVR YLDLSANVLK DEGLKTLCEA LKHPDCCLDS
841LCLVKCFITA AGCEDLASAL ISNQNLKILQ IGCNEIGDVG VQLLCRALTH TDCRLEILGL
901EECGLTSTCC KDLASVLTCS KTLQLLNLTL NTLDHTGVVV LCEALRHPEC ALQVLGLRKT
961DFDEETQALL TAEEERNPNL TITDDCDTIT RVEI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

994 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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