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Human MYO3B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human MYO3B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all MYO3B antibodies →
$220.00each
Catalog Number: LS-G88283-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Myosin IIIB(MYO3B) (NM_001083615) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MYO3B | Myosin IIIB | Myosin-IIIb
Origin Species
Human
NCBI GenBank
AK055778
SwissProt
Q8WXR4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
148.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKHLYGLFHY NPMMLGLESL PDPTDTWEII ETIGKGTYGK VYKVTNKRDG SLAAVKILDP
61VSDMDEEIEA EYNILQFLPN HPNVVKFYGM FYKADHCVGG QLWLVLELCN GGSVTELVKG
121LLRCGQRLDE AMISYILYGA LLGLQHLHNN RIIHRDVKGN NILLTTEGGV KLVDFGVSAQ
181LTSTRLRRNT SVGTPFWMAP EVIACEQQYD SSYDARCDVW SLGITAIELG DGDPPLFDMH
241PVKTLFKIPR NPPPTLLHPE KWCEEFNHFI SQCLIKDFER RPSVTHLLDH PFIKGVHGKV
301LFLQKQLAKV LQDQKHQNPV AKTRHERMHT RRPYHVEDAE KYCLEDDLVN LEVLDEDTII
361HQLQKRYADL LIYTYVGDIL IALNPFQNLS IYSPQFSRLY HGVKRASNPP HIFASADAAY
421QCMVTLSKDQ CIVISGESGS GKTESAHLIV QHLTFLGKAN NQTLREKILQ VNSLVEAFGN
481SCTAINDNSS RFGKYLEMMF TPTGVVMGAR ISEYLLEKSR VIKQAAREKN FHIFYYIYAG
541LHHQKKLSDF RLPEEKPPRY IADETGRVMH DITSKESYRR QFEAIQHCFR IIGFTDKEVH
601SVYRILAGIL NIGNIEFAAI SSQHQTDKSE VPNAEALQNA ASVLCISPEE LQEALTSHCV
661VTRGETIIRA NTVDRAADVR DAMSKALYGR LFSWIVNRIN TLLQPDENIC SAGGGMNVGI
721LDIFGFENFQ RNSFEQLCIN IANEQIQYYF NQHVFALEQM EYQNEGIDAV PVEYEDNRPL
781LDMFLQKPLG LLALLDEESR FPQATDQTLV DKFEDNLRCK YFWRPKGVEL CFGIQHYAGK
841VLYDASGVLE KNRDTLPADV VVVLRTSENK LLQQLFSIPL TKTGNLAQTR ARITVASSSL
901PPHFSAGKAK VDTLEVIRHP EETTNMKRQT VASYFRYSLM DLLSKMVVGQ PHFVRCIKPN
961DDREALQFSR ERVLAQLRST GILETVSIRR QGYSHRILFE EFVKRYYYLA FTAHQTPLAS
1021KESCVAILEK SRLDHWVLGK TKVFLKYYHV EQLNLLLREV IGRVVVLQAY TKGWLGARRY
1081KRVREKREKG AIAIQSGDTS NQSSGPHSPV AAGTRGSAEV QDCSEPGDHK VLRGSVHRRS
1141HSQAESNNGR TQTSSNSPAV TEKNGHSQAQ SSPKGCDIFA GHANKHSVSG TDLLSSRICH
1201PAPDQQGLSL WGAPQKPGSE NGLAQKHRTP RRRCQQPKML SSPEDTMYYN QLNGTLEYQG
1261SKRKPRKLGQ IKVLDGEDEY YKSLSPVDCI PEENNSAHPS FFSSSSKGDS FAQH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1314 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.