MYBPH Antibody
Catalog Number: LS-C500819
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general)
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MYBPH Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G77533-20
Catalog Number: LS-G77533
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MYBPH (NM_004997) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- H-protein | MYBPH | Myosin binding protein H | Myosin-binding protein H | MyBP-H
- Origin Species
- Human
- NCBI GenBank
- NM_004997
- SwissProt
- Q13203
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMEKNTSEGP ACSPEETASE SAKVPTAEPP GEVAVSESTR EEQVPKPQAP APQAPTASTA61TKPAPPSEDV PSAPLLLTLD DVSSSSVTVS WEPPERLGRL GLQGYVLELC REGASEWVPV121SARPMMVTQQ TVRNLALGDK FLLRVSAVSS AGAGPPAMLD QPIHIRENIE APKIRVPRHL181RQTYIRQVGE TVNLQIPFQG KPKPQATWTH NGHALDSQRV SMRTGDQDSI LFIRSAQRSD241SGRYELTVRV EDLEAKAVID ILVIEKPGPP SSIRLLDVWG CNAALQWTPP QDTGNTELLG301YMVQKADKKT GQWFTVLERY HPTTCTISDL IIGNSYSFRV FSENLCGLST SATVTKELAH361IQKADIAAKP KGFIERDFSE APSFTQPLAD HTSTPGYSTQ LFCSVRASPK PKIIWMKNKM421EIQGNPKYRA LSEQGVCTLE IRKPSPFDSG VYTCKAINVL GEASVDCRLE VKASAAHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
477 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
