Human METTL15 / METT5D1 Protein (Recombinant GST)
Catalog Number: LS-G36921
2 sizes / from $489.00
Expression System Tobacco / Plant-Based Expression
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human METTL15 / METT5D1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G93711-20
Catalog Number: LS-G93711
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human METT5D1(METTL15) (NM_001113528) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- METTL15 | Methyltransferase like 15 | METT5D1
- Origin Species
- Human
- SwissProt
- A6NJ78
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD QTQAQELHRS61QDRDFETMAK LHIPVMVDEV VHCLSPQKGQ IFLDMTFGSG GHTKAILQKE SDIVLYALDR121DPTAYALAEH LSELYPKQIR AMLGQFSQAE ALLMKAGVQP GTFDGVLMDL GCSSMQLDTP181ERGFSLRKDG PLDMRMDGGR YPDMPTAADV VNALDQQALA SILRTYGEEK HAKKIASAIV241QARSIYPITR TQQLASIVAG AFPPSAIYTR KDLLQRSTHI ATKTFQALRI FVNNELNELY301TGLKTAQKFL RPGGRLVALS FHSLEDRIVK RFLLGISMTE RFNLSVRQQV MKTSQLGSDH361ENTEEVSMRR APLMWELIHK KVLSPQDQDV QDNPRGRSAK LRAAIKLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
407 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
