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Human MEIS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human MEIS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G76607-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Meis homeobox 3(MEIS3) (NM_001009813) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Homeobox protein Meis3 | Meis homeobox 3 | MRG2 | Meis1-related protein 2 | MEIS3
Origin Species
Human
NCBI GenBank
AL359938
SwissProt
Q99687

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
38.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MARRYDELPH YPGIVDGPAA LASFPETVPA VPGPYGPHRP PQPLPPGLDS DGLKREKDEI
61YGHPLFPLLA LVFEKCELAT CSPRDGAGAG LGTPPGGDVC SSDSFNEDIA AFAKQVRSER
121PLFSSNPELD NLMIQAIQVL RFHLLELEKG KMPIDLVIED RDGGCREDFE DYPASCPSLP
181DQNNMWIRDH EDSGSVHLGT PGPSSGGLAS QSGDNSSDQG DGLDTSVASP SSGGEDEDLD
241QERRRNKKRG IFPKVATNIM RAWLFQHLSH PYPSEEQKKQ LAQDTGLTIL QVNNWFINAR
301RRIVQPMIDQ SNRTGQGAAF SPEGQPIGGY TETQPHVAVR PPGSVGMSLN LEGEWHYL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

358 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.