MDM2 Antibody (clone MDM2/2414)
Catalog Number: LS-C782570
2 sizes / from $379.00
Antibody: Mouse Monoclonal
Applications: Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MDM2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G87320-20
Catalog Number: LS-G87320
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MDM2 (NM_002392) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACTFS | Double minute 2 protein | Hdm2 | MDM2 variant FB30 | MDM2 | HDMX | Oncoprotein Mdm2 | p53-binding protein Mdm2 | MDM2 variant FB28
- Origin Species
- Human
- NCBI GenBank
- NM_002392
- SwissProt
- Q00987
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVRSRQMCNT NMSVPTDGAV TTSQIPASEQ ETLVRPKPLL LKLLKSVGAQ KDTYTMKEVL61FYLGQYIMTK RLYDEKQQHI VYCSNDLLGD LFGVPSFSVK EHRKIYTMIY RNLVVVNQQE121SSDSGTSVSE NRCHLEGGSD QKDLVQELQE EKPSSSHLVS RPSTSSRRRA ISETEENSDE181LSGERQRKRH KSDSISLSFD ESLALCVIRE ICCERSSSSE STGTPSNPDL DAGVSEHSGD241WLDQDSVSDQ FSVEFEVESL DSEDYSLSEE GQELSDEDDE VYQVTVYQAG ESDTDSFEED301PEISLADYWK CTSCNEMNPP LPSHCNRCWA LRENWLPEDK GKDKGEISEK AKLENSTQAE361EGFDVPDCKK TIVNDSRESC VEENDDKITQ ASQSQESEDY SQPSTSSSII YSSQEDVKEF421EREETQDKEE SVESSLPLNA IEPCVICQGR PKNGCIVHGK TGHLMACFTC AKKLKKRNKP481CPVCRQPIQM IVLTYFPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
497 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
