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Human MBD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human MBD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G84905-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MBD1 (NM_015845) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CXXC3 | MBD1 | RFT
Origin Species
Human
NCBI GenBank
NM_015846
SwissProt
Q9UIS9

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEDWLDCPA LGPGWKRREV FRKSGATCGR SDTYYQSPTG DRIRSKVELT RYLGPACDLT
61LFDFKQGILC YPAPKAHPVA VASKKRKKPS RPAKTRKRQV GPQSGEVRKE APRDETKADT
121DTAPASFPAP GCCENCGISF SGDGTQRQRL KTLCKDCRAQ RIAFNREQRM FKRVGCGECA
181ACQVTEDCGA CSTCLLQLPH DVASGLFCKC ERRRCLRIVE RSRGCGVCRG CQTQEDCGHC
241PICLRPPRPG LRRQWKCVQR RCLRGKHARR KGGCDSKMAA RRRPGAQPLP PPPPSQSPEP
301TEPQPYTNRR QNRKCGACAA CLRRMDCGRC DFCCDKPKFG GSNQKRQKCR WRQCLQFAMK
361RLLPSVWSES EDGAGSPPPY RRRKRPSSAR RHHLGPTLKP TLATRTAQPD HTQAPTKQEA
421GGGFVLPPPG TDLVFLREGA SSPVQVPGPV AASTEALLQA VDPGLPSVKQ EPPDPEEDKE
481ENKDDSASKL APEEEAGGAG TPVITEIFSL GGTRFRDTAV WLPSLQGRHS GREDGCKVWE
541TEDTVEPTST SWNPRGWPGT HVSLSPPPAS MMWVSCRRSW CPSSQS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

586 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.