MARCH9 Antibody
Catalog Number: LS-C497303
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MARCH9 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G87807-20
Catalog Number: LS-G87807
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MARCH9 (NM_138396) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RING finger protein 179 | RNF179 | MARCH-IX | MARCH9
- Origin Species
- Human
- NCBI GenBank
- NM_138396
- SwissProt
- Q86YJ5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLKSRLRMFL NELKLLVLTG GGRPRAEPQP RGGRGGGCGW APFAGCSTRD GDGDEEEYYG61SEPRARGLAG DKEPRAGPLP PPAPPLPPPG ALDALSLSSS LDSGLRTPQC RICFQGPEQG121ELLSPCRCDG SVRCTHQPCL IRWISERGSW SCELCYFKYQ VLAISTKNPL QWQAISLTVI181EKVQIAAIVL GSLFLVASIS WLIWSSLSPS AKWQRQDLLF QICYGMYGFM DVVCIGLIIH241EGSSVYRIFK RWQAVNQQWK VLNYDKTKDI GGDAGGGTAG KSGPRNSRTG PTSGATSRPP301AAQRMRTLLP QRCGYTILHL LGQLRPPDAR SSSHSGREVV MRVTTVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
346 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
