MAPK7 / ERK5 Antibody
Catalog Number: LS-C192874
2 sizes / from $364.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MAPK7 / ERK5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G75873-20
Catalog Number: LS-G75873
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ERK5(MAPK7) (NM_139032) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Big MAP kinase 1 | BMK-1 | Erk4 | ERK5 | MAPK 7 | MAPK7 | PRKM7 | BMK1 | BMK1 kinase | ERK-5 | MAP kinase 7
- Origin Species
- Human
- NCBI GenBank
- NM_002749
- SwissProt
- Q13164
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MESDLHQIIH SSQPLTLEHV RYFLYQLLRG LKYMHSAQVI HRDLKPSNLL VNENCELKIG61DFGMARGLCT SPAEHQYFMT EYVATRWYRA PELMLSLHEY TQAIDLWSVG CIFGEMLARR121QLFPGKNYVH QLQLIMMVLG TPSPAVIQAV GAERVRAYIQ SLPPRQPVPW ETVYPGADRQ181ALSLLGRMLR FEPSARISAA AALRHPFLAK YHDPDDEPDC APPFDFAFDR EALTRERIKE241AIVAEIEDFH ARREGIRQQI RFQPSLQPVA SEPGCPDVEM PSPWAPSGDC AMESPPPAPP301PCPGPAPDTI DLTLQPPPPV SEPAPPKKDG AISDNTKAAL KAALLKSLRS RLRDGPSAPL361EAPEPRKPVT AQERQREREE KRRRRQERAK EREKRRQERE RKERGAGASG GPSTDPLAGL421VLSDNDRSLL ERWTRMARPA APALTSVPAP APAPTPTPTP VQPTSPPPGP VAQPTGPQPQ481SAGSTSGPVP QPACPPPGPA PHPTGPPGPI PVPAPPQIAT STSLLAAQSL VPPPGLPGSS541TPGVLPYFPP GLPPPDAGGA PQSSMSESPD VNLVTQQLSK SQVEDPLPPV FSGTPKGSGA601GYGVGFDLEE FLNQSFDMGV ADGPQDGQAD SASLSASLLA DWLEGHGMNP ADIESLQREI661QMDSPMLLAD LPDLQDPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
677 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
