LSBio
Human MAGED2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human MAGED2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all MAGED2 antibodies →
$220.00each
Catalog Number: LS-G86246-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MAGED2 (NM_201222) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
11B6 | BCG-1 | BCG1 | HCA10 | MAGE-D2 | MAGE-D2 antigen | MAGED2 | Melanoma-associated antigen D2 | Melanoma antigen family D | 2 | JCL-1 | MAGED
Origin Species
Human
NCBI GenBank
NM_014599
SwissProt
Q9UNF1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDTSESGAG LTRFQAEASE KDSSSMMQTL LTVTQNVEVP ETPKASKALE VSEDVKVSKA
61SGVSKATEVS KTPEAREAPA TQASSTTQLT DTQVLAAENK SLAADTKKQN ADPQAVTMPA
121TETKKVSHVA DTKVNTKAQE TEAAPSQAPA DEPEPESAAA QSQENQDTRP KVKAKKARKV
181KHLDGEEDGS SDQSQASGTT GGRRVSKALM ASMARRASRG PIAFWARRAS RTRLAAWARR
241ALLSLRSPKA RRGKARRRAA KLQSSQEPEA PPPRDVALLQ GRANDLVKYL LAKDQTKIPI
301KRSDMLKDII KEYTDVYPEI IERAGYSLEK VFGIQLKEID KNDHLYILLS TLEPTDAGIL
361GTTKDSPKLG LLMVLLSIIF MNGNRSSEAV IWEVLRKLGL RPGIHHSLFG DVKKLITDEF
421VKQKYLDYAR VPNSNPPEYE FFWGLRSYYE TSKMKVLKFA CKVQKKDPKE WAAQYREAME
481ADLKAAAEAA AEAKARAEIR ARMGIGLGSE NAAGPCNWDE ADIGPWAKAR IQAGAEAKAK
541AQESGSASTG ASTSTNNSAS ASASTSGGFS AGASLTATLT FGLFAGLGGA GASTSGSSGA
601CGFSYK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

606 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.