LIMD1 Antibody (aa1-50)
Catalog Number: LS-C289878
2 sizes / from $301.00
Antibody: Rabbit Polyclonal
Applications: Immunoprecipitation
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human LIMD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G89905-20
Catalog Number: LS-G89905
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human LIMD1 (NM_014240) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LIMD1 | LIM domains containing 1
- Origin Species
- Human
- NCBI GenBank
- NM_014240
- SwissProt
- Q9UGP4
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDKYDDLGLE ASKFIEDLNM YEASKDGLFR VDKGAGNNPE FEETRRVFAT KMAKIHLQQQ61QQQLLQEETL PRGSRGPVNG GGRLGPQARW EVVGSKLTVD GAAKPPLAAS TGAPGAVTTL121AAGQPPYPPQ EQRSRPYLHG TRHGSQDCGS RESLATSEMS AFHQPGPCED PSCLTHGDYY181DNLSLASPKW GDKPGVSPSI GLSVGSGWPS SPGSDPPLPK PCGDHPLNHR QLSLSSSRSS241EGSLGGQNSG IGGRSSEKPT GLWSTASSQR VSPGLPSPNL ENGAPAVGPV QPRTPSVSAP301LALSCPRQGG LPRSNSGLGG EVSGVMSKPN VDPQPWFQDG PKSYLSSSAP SSSPAGLDGS361QQGAVPGLGP KPGCTDLGTG PKLSPTSLVH PVMSTLPELS CKEGPLGWSS DGSLGSVLLD421SPSSPRVRLP CQPLVPGPEL RPSAAELKLE ALTQRLEREM DAHPKADYFG ACVKCSKGVF481GAGQACQAMG NLYHDTCFTC AACSRKLRGK AFYFVNGKVF CEEDFLYSGF QQSADRCFLC541GHLIMDMILQ ALGKSYHPGC FRCVICNECL DGVPFTVDSE NKIYCVRDYH KVLAPKCAAC601GLPILPPEGS DETIRVVSMD RDYHVECYHC EDCGLELNDE DGHRCYPLED HLFCHSCHVK661RLEKRPSSTA LHQHHFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
676 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
