LEMD3 / MAN1 Antibody (clone OTI3H2)
Catalog Number: LS-C114870-100
100 µL (0.61 mg/mL) / $443.00
Antibody: Mouse Monoclonal
Applications: Immunofluorescence, Western Blot
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human LEMD3 / MAN1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G89932-20
Catalog Number: LS-G89932
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MAN1(LEMD3) (NM_001167614) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MAN1 | LEMD3 | LEM domain containing 3
- Origin Species
- Human
- NCBI GenBank
- NM_014319
- SwissProt
- Q9Y2U8
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 100.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAAASAPQ QLSDEELFSQ LRRYGLSPGP VTESTRPVYL KKLKKLREEE QQQHRSGGRG61NKTRNSNNNN TAAATVAAAG PAAAAAAGMG VRPVSGDLSY LRTPGGLCRI SASGPESLLG121GPGGASAAPA AGSKVLLGFS SDESDVEASP RDQAGGGGRK DRASLQYRGL KAPPAPLAAS181EVTNSNSAER RKPHSWWGAR RPAGPELQTP PGKDGAVEDE EGEGEDGEER DPETEEPLWA241SRTVNGSRLV PYSCRENYSD SEEEDDDDVA SSRQVLKDDS LSRHRPRRTH SKPLPPLTAK301SAGGRLETSV QGGGGLAMND RAAAAGSLDR SRNLEEAAAA EQGGGCDQVD SSPVPRYRVN361AKKLTPLLPP PLTDMDSTLD SSTGSLLKTN NHIGGGAFSV DSPRIYSNSL PPSAAVAASS421SLRINHANHT GSNHTYLKNT YNKPKLSEPE EELLQQFKRE EVSPTGSFSA HYLSMFLLTA481ACLFFLILGL TYLGMRGTGV SEDGELSKNP FGETFGKIQE SEKTLMMNTL YKLHDRLAQL541AGDHECGSSS QRTLSVQEAA AYLKDLGPEY EGIFNTSLQW ILENGKDVGI RCVGFGPEEE601LTNITDVQFL QSTRPLMSFW CRFRRAFVTV THRLLLLCLG VVMVCVVLRY MKYRWTKEEE661ETRQMYDMVV KIIDVLRSHN EACQENKDLQ PYMPIPHVRD SLIQPHDRKK MKKVWDRAVD721FLAANESRVR TETRRIGGAD FLVWRWIQPS ASCDKILVIP SKVWQGQAFH LDRRNSPPNS781LTPCLKIRNM FDPVMEIGDQ WHLAIQEAIL EKCSDNDGIV HIAVDKNSRE GCVYVKCLSP841EYAGKAFKAL HGSWFDGKLV TVKYLRLDRY HHRFPQALTS NTPLKPSNKH MNSMSHLRLR901TGLTNSQGSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
910 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
