LSBio
Human LDB3 / ZASP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human LDB3 / ZASP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G88258-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human LDB3 (NM_001080115) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CYPHER | KIAA0613 | KIAA01613 | LDB3 | LDB3Z4 | LIM domain binding 3 | LIM domain-binding protein 3 | LVNC3 | ORACLE | LDB3Z1 | ZASP | PDZ and LIM domain 6 | CMD1C | PDLIM6 | Protein cypher
Origin Species
Human
NCBI GenBank
AB014513
SwissProt
O75112

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
35.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MSYSVTLTGP GPWGFRLQGG KDFNMPLTIS RITPGSKAAQ SQLSQGDLVV AIDGVNTDTM
61THLEAQNKIK SASYNLSLTL QKSKRPIPIS TTAPPVQTPL PVIPHQKDPA LDTNGSLVAP
121SPSPEARASP GTPGTPELRP TFSPAFSRPS AFSSLAEASD PGPPRASLRA KTSPEGARDL
181LGPKALPGSS QPRQYNNPIG LYSAETLREM AQMYQMSLRG KASGVGLPGG SLPIKDLAVD
241SASPVYQAVI KSQNKPEDEA DEWARRSSNL QSRSFRILAQ MTGTEFMQDP DEEALRRSRE
301RFETERNSPR FAKLRNWHHG LSAQILNVKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

330 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.