LSBio
Human KRT79 / Keratin 79 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KRT79 / Keratin 79 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KRT79 antibodies →
$220.00each
Catalog Number: LS-G95462-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KRT6L(KRT79) (NM_175834) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CK-79 | Cytokeratin-79 | K79 | Keratin-6-like | Keratin-6L | Keratin 6L | Keratin 79 | KRT6L | KRT79 | Keratin-79 | Type-II keratin Kb38 | K6L | KB38
Origin Species
Human
NCBI GenBank
NM_175834
SwissProt
Q5XKE5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRSSVSRQTY STKGGFSSNS ASGGSGSQAR TSFSSVTVSR SSGSGGGAHC GPGTGGFGSR
61SLYNLGGHKS ISVSVAGGAL SGRALGGFGF GSRAFMGQGA GRQTFGPACP PGGIQEVTVN
121QSLLTPLHVE IDPEIQRVRT QEREQIKTLN NKFASFIDKV RFLEQQNKVL ETKWALLQEQ
181GQNLGVTRNN LEPLFEAYLG SMRSTLDRLQ SERGRLDSEL RNVQDLVEDF KNKYEDEINK
241HTAAENEFVV LKKDVDAAYM GRMDLHGKVG TLTQEIDFLQ QLYEMELSQV QTHVSNTNVV
301LSMDNNRNLD LDSIIAEVKA QYELIAQRSR AEAEAWYQTK YEELQVTAGK HGDNLRDTKN
361EIAELTRTIQ RLQGEADAAK KQCQQLQTAI AEAEQRGELA LKDAQKKLGD LDVALHQAKE
421DLTRLLRDYQ ELMNVKLALD VEIATYRKLL ESEESRMSGE CPSAVSISVT GNSTTVCGGG
481AASFGGGISL GGSGGATKGG FSTNVGYSTV KGGPVSAGTS ILRKTTTVKT SSQRY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

535 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.