LSBio
Human KRT71 / Keratin 71 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KRT71 / Keratin 71 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KRT71 antibodies →
$220.00each
Catalog Number: LS-G93288-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KRT71 (NM_033448) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cytokeratin-71 | CK-71 | HK6irs | Keratin-71 | Keratin 6 irs | KRT6IRS | KRT6IRS1 | KRT71 | HK6irs1 | K6IRS1 | K71 | KB34 | Keratin 71 | Type-II keratin Kb34
Origin Species
Human
NCBI GenBank
NM_033448
SwissProt
Q3SY84

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MSRQFTCKSG AAAKGGFSGC SAVLSGGSSS SFRAGSKGLS GGFGSRSLYS LGGVRSLNVA
61SGSGKSGGYG FGRGRASGFA GSMFGSVALG PVCPTVCPPG GIHQVTVNES LLAPLNVELD
121PEIQKVRAQE REQIKALNNK FASFIDKVRF LEQQNQVLET KWELLQQLDL NNCKNNLEPI
181LEGYISNLRK QLETLSGDRV RLDSELRNVR DVVEDYKKRY EEEINKRTAA ENEFVLLKKD
241VDAAYANKVE LQAKVESMDQ EIKFFRCLFE AEITQIQSHI SDMSVILSMD NNRNLDLDSI
301IDEVRTQYEE IALKSKAEAE ALYQTKFQEL QLAAGRHGDD LKNTKNEISE LTRLIQRIRS
361EIENVKKQAS NLETAIADAE QRGDNALKDA RAKLDELEGA LHQAKEELAR MLREYQELMS
421LKLALDMEIA TYRKLLESEE CRMSGEFPSP VSISIISSTS GGSVYGFRPS MVSGGYVANS
481SNCISGVCSV RGGEGRSRGS ANDYKDTLGK GSSLSAPSKK TSR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

523 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.