KRT4 / CK4 / Cytokeratin 4 Antibody
Catalog Number: LS-C813837
3 sizes / from $301.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human KRT4 / CK4 / Cytokeratin 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G76988-20
Catalog Number: LS-G76988
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Cytokeratin 4(KRT4) (NM_002272) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CK-4 | CK4 | Cytokeratin 4 | Cytokeratin-4 | K4 | Keratin 4 | Type-II keratin Kb4 | CYK4 | Keratin-4 | KRT4
- Origin Species
- Human
- NCBI GenBank
- X07695
- SwissProt
- P19013
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSVGVFSDM LNGCGKDGLV PRAKPRDVSD FSLYAPATKP CCSRTYKRRR LRAPALTGLG61PVTSLIAPSS LSAAMIARQQ CVRGGPRGFS CGSAIVGGGK RGAFSSVSMS GGAGRCSSGG121FGSRSLYNLR GNKSISMSVA GSRQGACFGG AGGFGTGGFG GGFGGSFSGK GGPGFPVCPA181GGIQEVTINQ SLLTPLHVEI DPEIQKVRTE EREQIKLLNN KFASFIDKVQ FLEQQNKVLE241TKWNLLQQQT TTTSSKNLEP LFETYLSVLR KQLDTLGNDK GRLQSELKTM QDSVEDFKTK301YEEEINKRTA AENDFVVLKK DVDAAYLNKV ELEAKVDSLN DEINFLKVLY DAELSQMQTH361VSDTSVVLSM DNNRNLDLDS IIAEVRAQYE EIAQRSKAEA EALYQTKVQQ LQISVDQHGD421NLKNTKSEIA ELNRMIQRLR AEIENIKKQC QTLQVSVADA EQRGENALKD AHSKRVELEA481ALQQAKEELA RMLREYQELM SVKLALDIEI ATYRKLLEGE EYRMSGECQS AVSISVVSGS541TSTGGISGGL GSGSGFGLSS GFGSGSGSGF GFGGSVSGSS SSKIISTTTL NKRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
594 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
