LSBio
Human KLHL42 / KLHDC5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KLHL42 / KLHDC5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KLHL42 antibodies →
$153.00each
Catalog Number: LS-G86226-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KLHDC5(KLHL42) (NM_020782) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ctb9 | KLHDC5 | Kelch domain containing 5 | KIAA1340
Origin Species
Human
NCBI GenBank
AB037761
SwissProt
Q9P2K6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAEEMVQIR LEDRCYPVSK RKLIEQSDYF RALYRSGMRE ALSQEAGGPE VQQLRGLSAP
61GLRLVLDFIN AGGAREGWLL GPRGEKGGGV DEDEEMDEVS LLSELVEAAS FLQVTSLLQL
121LLSQVRLNNC LEMYRLAQVY GLPDLQEACL RFMVVHFHEV LCKPQFHLLG SPPQAPGDVS
181LKQRLREARM TGTPVLVALG DFLGGPLAPH PYQGEPPSML RYEEMTERWF PLANNLPPDL
241VNVRGYGSAI LDNYLFIVGG YRITSQEISA AHSYNPSTNE WLQVASMNQK RSNFKLVAVN
301SKLYAIGGQA VSNVECYNPE QDAWNFVAPL PNPLAEFSAC ECKGKIYVIG GYTTRDRNMN
361ILQYCPSSDM WTLFETCDVH IRKQQMVSVE ETIYIVGGCL HELGPNRRSS QSEDMLTVQS
421YNTVTRQWLY LKENTSKSGL NLTCALHNDG IYIMSRDVTL STSLEHRVFL KYNIFSDSWE
481AFRRFPAFGH NLLVSSLYLP NKAET
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

505 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.