KLHL25 Antibody
Catalog Number: LS-C816603-100
100 µL / $309.00
Antibody: Rabbit Polyclonal
Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human KLHL25 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G86814-20
Catalog Number: LS-G86814
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KLHL25 (NM_022480) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BTB/POZ KELCH domain protein | ENC-2 | Ectodermal-neural cortex 2 | ENC2 | Kelch-like protein 25 | Kelch-like 25 (Drosophila) | KLHL25
- Origin Species
- Human
- NCBI GenBank
- NM_022480
- SwissProt
- Q9H0H3
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSVSVHETRK SRSSTGSMNV TLFHKASHPD CVLAHLNTLR KHCMFTDVTL WAGDRAFPCH61RAVLAASSRY FEAMFSHGLR ESRDDTVNFQ DNLHPEVLEL LLDFAYSSRI AINEENAESL121LEAGDMLQFH DVRDAAAEFL EKNLFPSNCL GMMLLSDAHQ CRRLYEFSWR MCLVHFETVR181QSEDFNSLSK DTLLDLISSD ELETEDERVV FEAILQWVKH DLEPRKVHLP ELLRSVRLAL241LPSDCLQEAV SSEALLMADE RTKLIMDEAL RCKTRILQND GVVTSPCARP RKAGHTLLIL301GGQTFMCDKI YQVDHKAKEI IPKADLPSPR KEFSASAIGC KVYVTGGRGS ENGVSKDVWV361YDTVHEEWSK AAPMLIARFG HGSAELENCL YVVGGHTSLA GVFPASPSVS LKQVEKYDPG421ANKWMMVAPL RDGVSNAAVV SAKLKLFVFG GTSIHRDMVS KVQCYDPSEN RWTIKAECPQ481PWRYTAAAVL GSQIFIMGGD TEFTAASAYR FDCETNQWTR IGDMTAKRMS CHALASGNKL541YVVGGYFGTQ RCKTLDCYDP TSDTWNCITT VPYSLIPTAF VSTWKHLPATRTRPLEQKLISEEDLAANDILDYKDDDDKV
589 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
