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Human KIAA1467 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KIAA1467 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KIAA1467 antibodies →
$153.00each
Catalog Number: LS-G82945-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1467(FAM234B) (NM_020853) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1467
Origin Species
Human
NCBI GenBank
AB040900
SwissProt
A2RU67

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67 kda
Protein Type
Lysate
Amino Acid Sequence
1MATVLSRALK LPGKKSPDLG EYDPLTQADS DESEDDLVLN LQKNGGVKNG KSPLGEAPEP
61DSDAEVAEAA KPHLSEVTTE GYPSEPLGGL EQKAASSLVS YVRTSVFLLT LGISMILVLL
121CAFLIPCPPR DLHSTWSRHL GSQGGGDLSP LELADVNGDG LRDVLLSFVM SRNGSAVGVS
181RPAANLVCLS GMNGSTLWSS LLPEEARDIT CLELMPGSLA ETICLVTGTH KMLSAFNATS
241GKAIWTLNPN YLSNGTLAAP VVVLPDLDED GVRDLVVLAI GELQPDLCFL LVSGRTGNPV
301GRPVKYNIVG VGNLIGPQVY ITTNGAVYIL FGFGNIQAVA LRDIFVQAQN RDSSPPSLQI
361EEPEWEKRRS INLSELIDVY SDGVELLQMV KAPDSNCSNL LITTRQSLVL LRGQNLTPYW
421ALRLQGLRSQ PTPGYFTDDQ TLDFLLQIQD GVGMKKMMVV DGDSGSIVWS YRAPCHMKET
481PATSAVTSDQ KSVFLFWAEG LSAASPNSDI ILGTEPPSLH HLYLLHPAFP SILLDLANTT
541GTVTASEVGI NDLWKDAFYV TRTTGPSSEG HPAALVVSKL SLRWALMEGQ MAQLQESTPK
601IGRGELRRFL SRIKFVEAPY EI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

622 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.