LSBio
Human KIAA1429 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KIAA1429 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KIAA1429 antibodies →
$220.00each
Catalog Number: LS-G82721-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1429 (NM_183009) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
AB037850

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
128.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVDSAMELL FLDTFKHPSA EQSSHIDVVR FPCVVYINEV RVIPPGVRAH SSLPDNRAYG
61ETSPHTFQLD LFFNNVSKPS APVFDRLGSL EYDENTSIIF RPNSKVNTDG LVLRGWYNCL
121TLAIYGSVDR VISHDRDSPP PPPPPPPPPQ PQPSLKRNPK HADGEKEDQF NGSPPRPQPR
181GPRTPPGPPP PDDDEDDPVP LPVSGDKEED APHREDYFEP ISPDRNSVPQ EGQYSDEGEV
241EEEQQEEGEE DEDDVDVEEE EDEDEDDRRT VDSIPEEEEE DEEEEGEEDE EGEGDDGYEQ
301ISSDEDGIAD LERETFKYPN FDVEYTAEDL ASVPPMTYDP YDRELVPLLY FSCPYKTTFE
361IEISRMKDQG PDKENSGAIE ASVKLTELLD LYREDRGAKW VTALEEIPSL IIKGLSYLQL
421KNTKQDSLGQ LVDWTMQALN LQVALRQPIA LNVRQLKAGT KLVSSLAECG AQGVTGLLQA
481GVISGLFELL FADHVSSSLK LNAFKALDSV ISMTEGMEAF LRGRQNEKSG YQKLLELILL
541DQTVRVVTAG SAILQKCHFY EVLSEIKRLG DHLAEKTSSL PNHSEPDHDT DAGLERTNPE
601YENEVEASMD MDLLESSNIS EGEIERLINL LEEVFHLMET APHTMIQQPV KSFPTMARIT
661GPPERDDPYP VLFRYLHSHH FLELVTLLLS IPVTSAHPGV LQATKDVLKF LAQSQKGLLF
721FMSEYEATNL LIRALCHFYD QDEEEGLQSD GVIDDAFALW LQDSTQTLQC ITELFSHFQR
781CTASEETDHS DLLGTLHNLY LITFNPVGRS AVGHVFSLEK NLQSLITLME YYSKEALGDS
841KSKKSVAYNY ACILILVVVQ SSSDVQMLEQ HAASLLKLCK ADENNAKLQE LGKWLEPLKN
901LRFEINCIPN LIEYVKQNID NLMTPEGVGL TTALRVLCNV ACPPPPVEGQ QKDLKWNLAV
961IQLFSAEGMD TFIRVLQKLN SILTQPWRLH VNMGTTLHRV TTISMARCTL TLLKTMLTEL
1021LRGGSFEFKD MRVPSALVTL HMLLCSIPLS GRLDSDEQKI QNDIIDILLT FTQGVNEKLT
1081ISEETLANNT WSLMLKEVLS SILKVPEGFF SGLILLSELL PLPLPMQTTQ VSLPYNMHLI
1141NDCSNTF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1147 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.