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Human KDM7A / JHDM1D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KDM7A / JHDM1D Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G87654-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human JHDM1D(KDM7A) (NM_030647) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1718 | Lysine-specific demethylase 7 | KDM7A | JHDM1D | KDM7
Origin Species
Human
NCBI GenBank
W96405
SwissProt
Q6ZMT4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
106.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGAAAAVAA GAAAGAAAAA VSVAAPGRAS APPPPPPVYC VCRQPYDVNR FMIECDICKD
61WFHGSCVGVE EHHAVDIDLY HCPNCAVLHG SSLMKKRRNW HRHDYTEIDD GSKPVQAGTR
121TFIKELRSRV FPSADEIIIK MHGSQLTQRY LEKHGFDVPI MVPKLDDLGL RLPSPTFSVM
181DVERYVGGDK VIDVIDVARQ ADSKMTLHNY VKYFMNPNRP KVLNVISLEF SDTKMSELVE
241VPDIAKKLSW VENYWPDDSV FPKPFVQKYC LMGVQDSYTD FHIDFGGTSV WYHVLWGEKI
301FYLIKPTDEN LARYESWSSS VTQSEVFFGD KVDKCYKCVV KQGHTLFVPT GWIHAVLTSQ
361DCMAFGGNFL HNLNIGMQLR CYEMEKRLKT PDLFKFPFFE AICWFVAKNL LETLKELRED
421GFQPQTYLVQ GVKALHTALK LWMKKELVSE HAFEIPDNVR PGHLIKELSK VIRAIEEENG
481KPVKSQGIPI VCPVSRSSNE ATSPYHSRRK MRKLRDHNVR TPSNLDILEL HTREVLKRLE
541MCPWEEDILS SKLNGKFNKH LQPSSTVPEW RAKDNDLRLL LTNGRIIKDE RQPFADQSLY
601TADSENEEDK RRTKKAKMKI EESSGVEGVE HEESQKPLNG FFTRVKSELR SRSSGYSDIS
661ESEDSGPECT ALKSIFTTEE SESSGDEKKQ EITSNFKEES NVMRNFLQKS QKPSRSEIPI
721KRECPTSTST EEEAIQGMLS MAGLHYSTCL QRQIQSTDCS GERNSLQDPS SCHGSNHEVR
781QLYRYDKPVE CGYHVKTEDP DLRTSSWIKQ FDTSRFHPQD LSRSQKCIRK EGSSEISQRV
841QSRNYVDSSG SSLQNGKYMQ NSNLTSGACQ ISNGSLSPER PVGETSFSVP LHPTKRPASN
901PPPISNQATK GKRPKKGMAT AKQRLGKILK LNRNGHARFF V
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

941 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.