LSBio
Human KCNT2 / KCa4.2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KCNT2 / KCa4.2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KCNT2 antibodies →
$220.00each
Catalog Number: LS-G93529-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KCNT2 (NM_198503) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KCa4.2 | KCNT2 | RP11-58O13.1 | SLICK | SLO2.1
Origin Species
Human
SwissProt
Q6UVM3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
130.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVDLESEVPP LPPRYRFRDL LLGDQGWQND DRVQVEFYMN ENTFKERLKL FFIKNQRSSL
61RIRLFNFSLK LLSCLLYIIR VLLENPSQGN EWSHIFWVNR SLPLWGLQVS VALISLFETI
121LLGYLSYKGN IWEQILRIPF ILEIINAVPF IISIFWPSLR NLFVPVFLNC WLAKHALENM
181INDLHRAIQR TQSAMFNQVL ILISTLLCLI FTCICGIQHL ERIGKKLNLF DSLYFCIVTF
241STVGFGDVTP ETWSSKLFVV AMICVALVVL PIQFEQLAYL WMERQKSGGN YSRHRAQTEK
301HVVLCVSSLK IDLLMDFLNE FYAHPRLQDY YVVILCPTEM DVQVRRVLQI PMWSQRVIYL
361QGSALKDQDL LRAKMDDAEA CFILSSRCEV DRTSSDHQTI LRAWAVKDFA PNCPLYVQIL
421KPENKFHIKF ADHVVCEEEF KYAMLALNCI CPATSTLITL LVHTSRGQEG QQSPEQWQKM
481YGRCSGNEVY HIVLEESTFF AEYEGKSFTY ASFHAHKKFG VCLIGVRRED NKNILLNPGP
541RYIMNSTDIC FYINITKEEN SAFKNQDQQR KSNVSRSFYH GPSRLPVHSI IASMGTVAID
601LQDTSCRSAS GPTLSLPTEG SKEIRRPSIA PVLEVADTSS IQTCDLLSDQ SEDETTPDEE
661MSSNLEYAKG YPPYSPYIGS SPTFCHLLHE KVPFCCLRLD KSCQHNYYED AKAYGFKNKL
721IIVAAETAGN GLYNFIVPLR AYYRPKKELN PIVLLLDNPP DMHFLDAICW FPMVYYMVGS
781IDNLDDLLRC GVTFAANMVV VDKESTMSAE EDYMADAKTI VNVQTLFRLF SSLSIITELT
841HPANMRFMQF RAKDCYSLAL SKLEKKERER GSNLAFMFRL PFAAGRVFSI SMLDTLLYQS
901FVKDYMISIT RLLLGLDTTP GSGFLCSMKI TADDLWIRTY ARLYQKLCSS TGDVPIGIYR
961TESQKLTTSE SQISISVEEW EDTKDSKEQG HHRSNHRNST SSDQSDHPLL RRKSMQWARR
1021LSRKGPKHSG KTAEKITQQR LNLYRRSERQ ELAELVKNRM KHLGLSTVGY DEMNDHQSTL
1081SYILINPSPD TRIELNDVVY LIRPDPLAYL PNSEPSRRNS ICNVTGQDSR EETQL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1135 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.