Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ITGBL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G79891-20
Catalog Number: LS-G79891
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human OSCP(ITGBL1) (NM_004791) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Integrin beta-like protein 1 | ITGBL1 | TIED | OSCP
- Origin Species
- Human
- NCBI GenBank
- NM_004791
- SwissProt
- O95965
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.92 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRPPGFRNFL LLASSLLFAG LSAVPQSFSP SLRSWPGAAC RLSRAESERR CRAPGQPPGA61ALCHGRGRCD CGVCICHVTE PGMFFGPLCE CHEWVCETYD GSTCAGHGKC DCGKCKCDQG121WYGDACQYPT NCDLTKKKSN QMCKNSQDII CSNAGTCHCG RCKCDNSDGS GLVYGKFCEC181DDRECIDDET EEICGGHGKC YCGNCYCKAG WHGDKCEFQC DITPWESKRR CTSPDGKICS241NRGTCVCGEC TCHDVDPTGD WGDIHGDTCE CDERDCRAVY DRYSDDFCSG HGQCNCGRCD301CKAGWYGKKC EHPQSCTLSA EESIRKCQGS SDLPCSGRGK CECGKCTCYP PGDRRVYGKT361CECDDRRCED LDGVVCGGHG TCSCGRCVCE RGWFGKLCQH PRKCNMTEEQ SKNLCESADG421ILCSGKGSCH CGKCICSAEE WYISGEFCDC DDRDCDKHDG LICTGNGICS CGNCECWDGW481NGNACEIWLG SEYPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
494 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
