ISM1 Antibody
Catalog Number: LS-C830186
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ISM1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95116-20
Catalog Number: LS-G95116
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ISM1 (NM_080826) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA149I18.1 | C20orf82 | Isthmin-1 | ISM1 | Isthmin 1 homolog (zebrafish) | DJ1077I2.1 | ISM | Isthmin
- Origin Species
- Human
- NCBI GenBank
- NM_080826
- SwissProt
- B1AKI9
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVRLAAELLL LLGLLLLTLH ITVLRGSGAA DGPDAAAGNA SQAQLQNNLN VGSDTTSETS61FSLSKEAPRE HLDHQAAHQP FPRPRFRQET GHPSLQRDFP RSFLLDLPNF PDLSKADING121QNPNIQVTIE VVDGPDSEAD KDQHPENKPS WSVPSPDWRA WWQRSLSLAR ANSGDQDYKY181DSTSDDSNFL NPPRGWDHTA PGHRTFETKD QPEYDSTDGE GDWSLWSVCS VTCGNGNQKR241TRSCGYACTA TESRTCDRPN CPGIEDTFRT AATEVSLLAG SEEFNATKLF EVDTDSCERW301MSCKSEFLKK YMHKVMNDLP SCPCSYPTEV AYSTADIFDR IKRKDFRWKD ASGPKEKLEI361YKPTARYCIR SMLSLESTTL AAQHCCYGDN MQLITRGKGA GTPNLISTEF SAELHYKVDV421LPWIICKGDW SRYNEARPPN NGQKCTESPS DEDYIKQFQE AREYSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
464 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
