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Human IQCJ Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human IQCJ Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all IQCJ Proteins & Peptides →
$153.00each
Catalog Number: LS-G95114-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SCHIP1(IQCJ) (NM_001197114) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Calmodulin binding protein | IQ domain-containing protein J | IQCJ | IQ motif containing J
Origin Species
Hu
NCBI GenBank
NM_001042705
SwissProt
Q1A5X6

Protein

Expression System
HEK293T
Fusion Tag
FLAG, Myc
Molecular Weight
59.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRLEELKRLQ NPLEQVNDGK YSFENIQRAW REYLQRQEPL GKRSPSPPSV SSEKLSSSVS
61MNTFSDSSTP DYREDGMDLG SDAGSSSSSS RASSQSNSTK VTPCSECKSS SSPGGSLDLV
121SALEDYEEPF PVYQKKVIDE WAPEEDGEEE EEEDERDQRG YRDDRSPARE PGDVSARTRS
181GGGGGRSATT AMPPPVPNGN LHQHDPQDLR HNGNVVVAGR PSCSRGPRRA IQKPQPAGGR
241RSGRGPAAGG LCLQPPDGGT CVPEEPPVPP MDWEALEKHL AGLQFREQEV RNQGQARTNS
301TSAQKNERES IRQKLALGSF FDDGPGIYTS CSKSGKPSLS SRLQSGMNLQ ICFVNDSGSD
361KDSDADDSKT ETSLDTPLSP MSKQSSSYSD RDTTEEESES LDDMDFLTRQ KKLQAEAKMA
421LAMAKPMAKM QVEVEKQNRK KSPVADLLPH MPHISECLMK RSLKPTDLRD MTIGQLQVIV
481NDLHSQIESL NEELVQLLLI RDELHTEQDA MLVDIEDLTR HAESQQKHMA EKMPAK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

536 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.