LSBio
Human IL17REL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human IL17REL Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all IL17REL antibodies →
$153.00each
Catalog Number: LS-G96274-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human IL17REL (NM_001001694) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001001694

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
37.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MSRSVLEALT SSTAMQCVPS DGCAMLLRVR ASITLHERLR GLEACAMSLD TQETQCQSVW
61VARASHRQQG GQQLQVHFGC FAVSVAQHLY VTLRTIPHFC GVQLDQRHLV EAGKLSYWVD
121RRRKAILVQV PRASGSPDYY LRLCLKRFTC EDAGAPVRVT ANSVSQAVFL PYSQELPCLC
181LEGWSATPDA VRIQICPFEN DTEALEVLWD TVYYHPESQT LSWEPACPVS GHVSLCWRPG
241PGAGCRKLQQ SSQLVHRRVQ YPLVDTQPQL CLKFSTSWGS WVRCPFEQRR FPTPPTSRCT
301CVTGGSHSSL PANAHSRPAR SLQPQVTWQP PLLLPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

336 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.