LSBio
Human IL12B + IL23A Heterodimer Protein (Recombinant)

Human IL12B + IL23A Heterodimer Protein (Recombinant)

ProteinLSBio Guarantee
Expression SystemOther
Format:Lyophilized
$293.00each
Catalog Number: LS-G98031-5

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Overview

Description
The recombinant IL23 heterodimer of human IL23A/IL12B comprises 498 (181 + 317) amino acids and has a calculated molecular mass of 56.3 (20.1 + 36.2) kDa. The apparent molecular mass of rhIL23 heterodimer is approximately 22 & 45 kDa respectively in SDS-PAGE under reducing conditions. A DNA sequence encoding the IL23A (Q9NPF7) (Met 1-Pro 189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL23 heterodimer was purified.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Origin Species
Human

Protein

Expression System
Other
Molecular Weight
56.3 kDa
Protein Type
Recombinant
Amino Acid Sequence
1Met 1-Pro  189 and Me t 1-Ser 32 8

31 aa

Format

Purity
>90%
Conjugate/Label
Unconjugated
Endotoxin Level
< 1.0 EU/µg
Format
Lyophilized
Formulation
Lyophilized from sterile PBS, pH 7.4
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Store at -70°C.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.