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Human HTR7 / 5HT7 Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human HTR7 / 5HT7 Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all HTR7 antibodies →
$153.00each
Catalog Number: LS-G74147-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human 5HT7 Receptor(HTR7) (NM_019860) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
5-HT7 | 5-HT7 receptor | 5-HT-7 | 5-hydroxytryptamine 7 receptor | 5HT7 | 5-HT-X | 5-hydroxytryptamine receptor 7 | 5HT7 Receptor | HT7 | Serotonin 5-HT7 receptor | Serotonin receptor 7 | HTR7
Origin Species
Human
NCBI GenBank
NM_019859
SwissProt
P34969

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
47.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MMDVNSSGRP DLYGHLRSFL LPEVGRGLPD LSPDGGADPV AGSWAPHLLS EVTASPAPTW
61DAPPDNASGC GEQINYGRVE KVVIGSILTL ITLLTIAGNC LVVISVCFVK KLRQPSNYLI
121VSLALADLSV AVAVMPFVSV TDLIGGKWIF GHFFCNVFIA MDVMCCTASI MTLCVISIDR
181YLGITRPLTY PVRQNGKCMA KMILSVWLLS ASITLPPLFG WAQNVNDDKV CLISQDFGYT
241IYSTAVAFYI PMSVMLFMYY QIYKAARKSA AKHKFPGFPR VEPDSVIALN GIVKLQKEVE
301ECANLSRLLK HERKNISIFK REQKAATTLG IIVGAFTVCW LPFFLLSTAR PFICGTSCSC
361IPLWVERTFL WLGYANSLIN PFIYAFFNRD LRTTYRSLLQ CQYRNINRKL SAAGMHEALK
421LAERPERPEF VLQNADYCRK KGHDS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

445 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.