HNF4G / HNF4 Gamma Antibody
Catalog Number: LS-C487000-100
100 µL / $309.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human HNF4G / HNF4 Gamma Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G84496-20
Catalog Number: LS-G84496
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human HNF4 gamma(HNF4G) (NM_004133) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HNF-4-gamma | HNF4-beta | HNF4 gamma | Hnf4gamma | HNF4G | NR2A3 | NR2A2
- Origin Species
- Human
- NCBI GenBank
- NM_004133
- SwissProt
- Q14541
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDMANYSEVL DPTYTTLEFE TMQILYNSSD SSAPETSMNT TDNGVNCLCA ICGDRATGKH61YGASSCDGCK GFFRRSIRKS HVYSCRFSRQ CVVDKDKRNQ CRYCRLRKCF RAGMKKEAVQ121NERDRISTRR STFDGSNIPS INTLAQAEVR SRQISVSSPG SSTDINVKKI ASIGDVCESM181KQQLLVLVEW AKYIPAFCEL PLDDQVALLR AHAGEHLLLG ATKRSMMYKD ILLLGNNYVI241HRNSCEVEIS RVANRVLDEL VRPFQEIQID DNEYACLKAI VFFDPDAKGL SDPVKIKNMR301FQVQIGLEDY INDRQYDSRG RFGELLLLLP TLQSITWQMI EQIQFVKLFG MVKIDNLLQE361MLLGGASNDG SHLHHPMHPH LSQDPLTGQT ILLGPMSTLV HADQISTPET PLPSPPQGSG421QEQYKIAANQ ASVISHQHLS KQKQLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
445 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
