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Human HMMR / CD168 / RHAMM Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human HMMR / CD168 / RHAMM Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all HMMR antibodies →
$220.00each
Catalog Number: LS-G81503-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CD168(HMMR) (NM_012485) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CD168 | CD168 antigen | HMMR | RHAMM | IHABP
Origin Species
Human
NCBI GenBank
NM_012484
SwissProt
O75330

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSFPKAPLKR FNDPSGCAPS PGAYDVKTLE VLKGPVSFQK SQRFKQQKES KQNLNVDKDT
61TLPASARKVK SSESKIRVLL QERGAQDRRI QDLETELEKM EARLNAALRE KTSLSANNAT
121LEKQLIELTR TNELLKSKFS ENGNQKNLRI LSLELMKLRN KRETKMRGMM AKQEGMEMKL
181QVTQRSLEES QGKIAQLEGK LVSIEKEKID EKSETEKLLE YIEEISCASD QVEKYKLDIA
241QLEENLKEKN DEILSLKQSL EENIVILSKQ VEDLNVKCQL LEKEKEDHVN RNREHNENLN
301AEMQNLKQKF ILEQQEREKL QQKELQIDSL LQQEKELSSS LHQKLCSFQE EMAKEKNLFE
361EELKQTLDEL DKLQQKEEQA ERLVKQLEEE AKSRAEELKL LEEKLKGKEA ELEKSSAAHT
421QATLLLQEKY DSMVQSLEDV TAQFESYKAL TASEIEDLKL ENSSLQEKAA KAGKNAEDVQ
481HQILATESSN QEYVRMLLDL QTKSALKETE IKEITVSFLQ KITDLQNQLK QQEEDFRKQL
541EDEEGRKAEK ENTTAELTEE INKWRLLYEE LYNKTKPFQL QLDAFEVEKQ ALLNEHGAAQ
601EQLNKIRDSY AKLLGHQNLK QKIKHVVKLK DENSQLKSEV SKLRCQLAKK KQSETKLQEE
661LNKVLGIKHF DPSKAFHHES KENFALKTPL KEGNTNCYRA PMECQESWK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

709 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.