Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human HLA-DQB3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G81291-20
Catalog Number: LS-G81291
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human HLA (NM_182549) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- D6S205 | D6S205E | DVbeta | DQB3 | HLA-DVB | HLA-DQB3 | DVB
- Origin Species
- Human
- NCBI GenBank
- NM_002123
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 27 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSWKMALQIP GGFWAAAVTV MLVMLSTPVA EARDFPKDFL VQFKGMCYFT NGTERVRGVA61RYIYNREEYG RFDSDVGEFQ AVTELGRSIE DWNNYKDFLE QERAAVDKVC RHNYEAELRT121TLQRQVEPTV TISPSRTEAL NHHNLLVCSV TDFYPAQIKV QWFRNDQEET AGVVSTSLIR181NGDWTFQILV MLEITPQRGD IYTCQVEHPS LQSPITVEWR PRGPPPAGLL HTRTRPLEQKLISEEDLAANDILDYKDDDDKV
231 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.