HLA-B Antibody
Catalog Number: LS-C24466-100
100 µL / $1248.00
Antibody: Mouse Monoclonal
Applications: Cytotoxicity Assay, Flow Cytometry
Reactivity: Human
Format: Lyophilized
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human HLA-B Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G87914-20
Catalog Number: LS-G87914
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human HLAB(HLA) (NM_005514) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HLAB | MHC Class I HLA heavy chain | Leukocyte antigen class I-B | SPDA1
- Origin Species
- Human
- NCBI GenBank
- NM_005514
- SwissProt
- P01889
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.33 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLVMAPRTVL LLLSAALALT ETWAGSHSMR YFDTAMSRPG RGEPRFISVG YVDDTQFVRF61DSDAASPREE PRAPWIEQEG PEYWDRNTQI FKTNTQTDRE SLRNLRGYYN QSEAGSHTLQ121SMYGCDVGPD GRLLRGHNQY AYDGKDYIAL NEDLRSWTAA DTAAQITQRK WEAARVAEQD181RAYLEGTCVE WLRRYLENGK DTLERADPPK THVTHHPISD HEATLRCWAL GFYPAEITLT241WQRDGEDQTQ DTELVETRPA GDRTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP301SSQSTVPIVG IVAGLAVLAV VVIGAVVAAV MCRRKSSGGK GGSYSQAACS DSAQGSDVSL361TATRTRPLEQKLISEEDLAANDILDYKDDDDKV
362 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
