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Human HERC6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human HERC6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all HERC6 antibodies →
$153.00each
Catalog Number: LS-G82279-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human HERC6 (NM_017912) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Hect domain and RLD 6 | HERC6 | Potential ubiquitin ligase
Origin Species
Human
NCBI GenBank
AK000644
SwissProt
Q8IVU3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
114.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYFCWGADSR ELQRRRTAGS PGAELLQAAS GERHSLLLLT NHRVLSCGDN SRGQLGRRGA
61QRGELPEPIQ ALETLIVDLV SCGKEHSLAV CHKGRVFAWG AGSEGQLGIG EFKEISFTPK
121KIMTLNDIKI IQVSCGHYHS LALSKDSQVF SWGKNSHGQL GLGKEFPSQA SPQRVRSLEG
181IPLAQVAAGG AHSFALSLCG TSFGWGSNSA GQLALSGRNV PVQSNKPLSV GALKNLGVVY
241ISCGDAHTAV LTQDGKVFTF GDNRSGQLGY SPTPEKRGPQ LVERIDGLVS QIDCGSYHTL
301AYVHTTGQVV SFGHGPSDTS KPTHPEALTE NFDISCLISA EDFVDVQVKH IFAGTYANFV
361TTHQDTSSTR APGKTLPEIS RISQSMAEKW IAVKRRSTEH EMAKSEIRMI FSSPACLTAS
421FLKKRGTGET TSIDVDLEMA RDTFKKLTKK EWISSMITTC LEDDLLRALP CHSPHQEALS
481VFLLLPECPV MHDSKNWKNL VVPFAKAVCE MSKQSLQVLK KCWAFLQESS LNPLIQMLKA
541AIISQLLHQT KTEQDHCNVK ALLGMMKELH KVNKANCRLP ENTFNINELS NLLNFYIDRG
601RQLFRDNHLI PAETPSPVIF SDFPFIFNSL SKIKLLQADS HIKMQMSEKK AYMLMHETIL
661QKKDEFPPSP RFILRVRRSR LVKDALRQLS QAEATDFCKV LVVEFINEIC PESGGVSSEF
721FHCMFEEMTK PEYGMFMYPE MGSCMWFPAK PKPEKKRYFL FGMLCGLSLF NLNVANLPFP
781LALYKKLLDQ KPSLEDLKEL SPRLGKSLQE VLDDAADDIG DALCIRFSIH WDQNDVDLIP
841NGISIPVDQT NKRDYVSKYI DYIFNVSVKA VYEEFQRGFY RVCEKEILRH FYPEELMTAI
901IGNTDYDWKQ FEQNSKYEQG YQKSHPTIQL FWKAFHKLTL DEKKKFLFFL TGRDRLHARG
961IQKMEIVFRC PETFSERDHP TSITCHNILS LPKYSTMERM EEALQVAINN NRGFVSPMLT
1021QS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1022 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.