HELT Antibody
Catalog Number: LS-C666111-100
100 µg / $348.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human HELT Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G93365-20
Catalog Number: LS-G93365
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human HELT (NM_001029887) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BHLHb44 | Helt bHLH transcription factor | Mgn | HCM1228 | Hey-like transcription factor | MEGANE | HELT | HESL | Heslike | Megane bHLH factor
- Origin Species
- Human
- NCBI GenBank
- NM_001029887
- SwissProt
- A6NFD8
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDKLKERKV SRLSPNGTCA LVVEASDSPT RHLGGPMAGK CPHGTLSVEE SRVRTPVSHK61VIEKRRRDRI NRCLNELGKT VPMALAKQGE PQEALAQIRS RVRSLVLSSA TVPDQQALGR121CEGPFLLLQS SGKLEKAEIL EMTVQYLRAL HSADFPRGRE KAELLAEFAN YFHYGYHECM181KNLVHYLTTV ERMETKDTKY ARILAFLQSK ARLGAEPAFP PLGSLPEPDF SYQLHPAGPE241FAGHSPGEAA VFPQGSGAGP FPWPPGAARS PALPYLPSAP VPLASPAQQH SPFLTPVQGL301DRHYLNLIGH AHPNALNLHT PQHPPVLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
327 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
