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Human GZF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GZF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G87587-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZNF336(GZF1) (NM_022482) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ322G13.2 | GZF1 | ZBTB23 | Zinc finger protein 336 | ZNF336
Origin Species
Human
NCBI GenBank
AK025447

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESGAVLLES KSSPFNLLHE MHELRLLGHL CDVTVSVEYQ GVRKDFMAHK AVLAATSKFF
61KEVFLNEKSV DGTRTNVYLN EVQVADFASF LEFVYTAKVQ VEEDRVQRML EVAEKLKCLD
121LSETCFQLKK QMLESVLLEL QNFSESQEVE VSSGSQVSAA PAPRASVATD GPHPSGLTDS
181LDYPGERASN GMSSDLPPKK SKDKLDKKKE VVKPPYPKIR RASGRLAGRK VFVEIPKKKY
241TRRLREQQKT AEGDVGDYRC PQDQSPDRVG TEMEPVSKNE GCQAGAELEE LSKKAGPEEE
301EEEEEEDEEG EKKKSNFKCS ICEKAFLYEK SFLKHSKHRH GVATEVVYRC DTCGQTFANR
361CNLKSHQRHV HSSERHFPCE LCGKKFKRKK DVKRHVLQVH EGGGERHRCG QCGKGLSSKT
421ALRLHERTHT GDRPYGCTEC GARFSQPSAL KTHMRIHTGE KPFVCDECGA RFTQNHMLIY
481HKRCHTGERP FMCETCGKSF ASKEYLKHHN RIHTGSKPFK CEVCFRTFAQ RNSLYQHIKV
541HTGERPYCCD QCGKQFTQLN ALQRHRRIHT GERPFMCNAC GRTFTDKSTL RRHTSIHDKN
601TPWKSFLVIV DGSPKNDDGH KTEQPDEEYV SSKLSDKLLS FAENGHFHNL AAVQDTVPTM
661QENSSADTAC KADDSVVSQD TLLATTISEL SELTPQTDSM PTQLHSLSNM E
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

711 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.